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t7 oligo dt promoter primer  (Thermo Fisher)


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    Thermo Fisher t7 oligo dt promoter primer
    T7 Oligo Dt Promoter Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/t7+oligo+dt+promoter+primer/Oligomycin/us12023339-441-21-24
    Average 99 stars, based on 1 article reviews
    t7 oligo dt promoter primer - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Purification:

    Article Title: Translation Elongation Factor eEF1A2 is a Novel Anticancer Target for the Marine Natural Product Plitidepsin
    Article Snippet: Total RNA was obtained from four samples of each HeLa and HeLa-APL-R cells with TRIzol reagent (Life technologies) under the recommendations of the manufacturer. .. Once purified, 15 μg RNA from each sample were reverse transcribed with a T7-Oligo(dT) promoter primer in the first-strand cDNA synthesis reaction, as indicated in the GeneChip One-Cycle Target Labeling Kit protocol (Affymetrix, Santa Clara, CA, USA). ..

    Article Title: Methods, compositions and devices for treating cancer with illudofulvenes
    Article Snippet: Prior to use in a Microarray analysis, the purified mRNA ( ̃1 to 2 μg) can be first reverse transcribed using a T7-Oligo(dT) Promoter Primer (Affymetrix GeneChip T7-Oligo(dT) Promoter Primer Kit 900375, Waltham, MA) in the first-strand cDNA synthesis reaction. .. Prior to use in a Microarray analysis, the purified mRNA ( ̃1 to 2 μg) can be first reverse transcribed using a T7-Oligo(dT) Promoter Primer (Affymetrix GeneChip T7-Oligo(dT) Promoter Primer Kit 900375, Waltham, MA) in the first-strand cDNA synthesis reaction. .. Next an RNase H-mediated second-strand cDNA synthesis can be performed, the double-stranded cDNA can be purified, and this double-strand cDNA serves as the template in a subsequent in vitro transcription (IVT) reaction (Affymetrix GeneChip IVT kit 900688, Waltham, MA).

    Article Title: Methods, compositions and devices for treating cancer with illudofulvenes
    Article Snippet: Prior to use in a Microarray analysis, the purified mRNA ( ̃1 to 2 μg) can be first reverse transcribed using a T7-Oligo(dT) Promoter Primer (Affymetrix GeneChip T7-Oligo(dT) Promoter Primer Kit 900375, Waltham, Mass.) in the first-strand cDNA synthesis reaction. .. Prior to use in a Microarray analysis, the purified mRNA ( ̃1 to 2 μg) can be first reverse transcribed using a T7-Oligo(dT) Promoter Primer (Affymetrix GeneChip T7-Oligo(dT) Promoter Primer Kit 900375, Waltham, Mass.) in the first-strand cDNA synthesis reaction. .. Next an RNase H-mediated second-strand cDNA synthesis can be performed, the double-stranded cDNA can be purified, and this double-strand cDNA serves as the template in a subsequent in vitro transcription (IVT) reaction (Affymetrix GeneChip IVT kit 900688, Waltham, Mass.).

    Reverse Transcription:

    Article Title: Translation Elongation Factor eEF1A2 is a Novel Anticancer Target for the Marine Natural Product Plitidepsin
    Article Snippet: Total RNA was obtained from four samples of each HeLa and HeLa-APL-R cells with TRIzol reagent (Life technologies) under the recommendations of the manufacturer. .. Once purified, 15 μg RNA from each sample were reverse transcribed with a T7-Oligo(dT) promoter primer in the first-strand cDNA synthesis reaction, as indicated in the GeneChip One-Cycle Target Labeling Kit protocol (Affymetrix, Santa Clara, CA, USA). ..

    Article Title: Expression of Ten-m/Odz3 in the fibrous layer of mandibular condylar cartilage during postnatal growth in mice
    Article Snippet: .. Total RNA was mixed with T7-oligo (dT) promoter primer, reverse-transcribed to cDNA, and synthesized to double-stranded cDNA with a GeneChip® Expression 3′-Amplification Reagents Two-Cycle cDNA Synthesis Kit (Affymetrix, Santa Clara, CA, USA). .. The double-stranded cDNA was purified and served as a template in the subsequent in-vitro transcription using a GeneChip® in-vitro transcription labeling kit (Affymetrix).

    Article Title: Evaluation of in vitro macrophage differentiation during space flight
    Article Snippet: .. Briefly, the two round RNA amplification and labeling procedure was performed using the Affymetrix Small Sample Labeling Protocol vII (2xIVT) as follows: 50 ng of total RNA was primed with T7-oligo(dT) promoter primer (Affymetrix, Santa Clara, CA) and reverse transcribed using SuperScript Reverse Transcriptase vII kit (Invitrogen). .. The first round of RNA amplification was performed on the cDNA using the MEGAScript T7 Invitro-Transciption kit (Ambion).

    Article Title: Methods, compositions and devices for treating cancer with illudofulvenes
    Article Snippet: Prior to use in a Microarray analysis, the purified mRNA ( ̃1 to 2 μg) can be first reverse transcribed using a T7-Oligo(dT) Promoter Primer (Affymetrix GeneChip T7-Oligo(dT) Promoter Primer Kit 900375, Waltham, MA) in the first-strand cDNA synthesis reaction. .. Prior to use in a Microarray analysis, the purified mRNA ( ̃1 to 2 μg) can be first reverse transcribed using a T7-Oligo(dT) Promoter Primer (Affymetrix GeneChip T7-Oligo(dT) Promoter Primer Kit 900375, Waltham, MA) in the first-strand cDNA synthesis reaction. .. Next an RNase H-mediated second-strand cDNA synthesis can be performed, the double-stranded cDNA can be purified, and this double-strand cDNA serves as the template in a subsequent in vitro transcription (IVT) reaction (Affymetrix GeneChip IVT kit 900688, Waltham, MA).

    Article Title: Methods, compositions and devices for treating cancer with illudofulvenes
    Article Snippet: Prior to use in a Microarray analysis, the purified mRNA ( ̃1 to 2 μg) can be first reverse transcribed using a T7-Oligo(dT) Promoter Primer (Affymetrix GeneChip T7-Oligo(dT) Promoter Primer Kit 900375, Waltham, Mass.) in the first-strand cDNA synthesis reaction. .. Prior to use in a Microarray analysis, the purified mRNA ( ̃1 to 2 μg) can be first reverse transcribed using a T7-Oligo(dT) Promoter Primer (Affymetrix GeneChip T7-Oligo(dT) Promoter Primer Kit 900375, Waltham, Mass.) in the first-strand cDNA synthesis reaction. .. Next an RNase H-mediated second-strand cDNA synthesis can be performed, the double-stranded cDNA can be purified, and this double-strand cDNA serves as the template in a subsequent in vitro transcription (IVT) reaction (Affymetrix GeneChip IVT kit 900688, Waltham, Mass.).

    Article Title: Inhibitors of Differentiation (ID1, ID2, ID3 and ID4) genes are neuronal targets of MeCP2 that are elevated in Rett syndrome
    Article Snippet: A clean up of RNA was performed using RNeasy Mini kit (QIAGEN) before proceeding to cDNA synthesis. .. 1 μg of RNA under each condition was first reverse transcribed using T7-oligo (dT) promoter primer followed by RNase-H mediated second-stranded cDNA synthesis using One-Cycle cDNA Synthesis kit (Affymetrix). ..

    cDNA Synthesis:

    Article Title: Translation Elongation Factor eEF1A2 is a Novel Anticancer Target for the Marine Natural Product Plitidepsin
    Article Snippet: Total RNA was obtained from four samples of each HeLa and HeLa-APL-R cells with TRIzol reagent (Life technologies) under the recommendations of the manufacturer. .. Once purified, 15 μg RNA from each sample were reverse transcribed with a T7-Oligo(dT) promoter primer in the first-strand cDNA synthesis reaction, as indicated in the GeneChip One-Cycle Target Labeling Kit protocol (Affymetrix, Santa Clara, CA, USA). ..

    Article Title: Expression of Ten-m/Odz3 in the fibrous layer of mandibular condylar cartilage during postnatal growth in mice
    Article Snippet: .. Total RNA was mixed with T7-oligo (dT) promoter primer, reverse-transcribed to cDNA, and synthesized to double-stranded cDNA with a GeneChip® Expression 3′-Amplification Reagents Two-Cycle cDNA Synthesis Kit (Affymetrix, Santa Clara, CA, USA). .. The double-stranded cDNA was purified and served as a template in the subsequent in-vitro transcription using a GeneChip® in-vitro transcription labeling kit (Affymetrix).

    Article Title: Precision Profiling and Components of Variability Analysis for Affymetrix Microarray Assays Run in a Clinical Context
    Article Snippet: .. Each total RNA sample was then used for a single cDNA synthesis reaction, using the Affymetrix T7-Oligo (dT) promoter primer, which was subsequently transcribed, labeled, fragmented, and hybridized to one U133A array. ..

    Article Title: Methods, compositions and devices for treating cancer with illudofulvenes
    Article Snippet: Prior to use in a Microarray analysis, the purified mRNA ( ̃1 to 2 μg) can be first reverse transcribed using a T7-Oligo(dT) Promoter Primer (Affymetrix GeneChip T7-Oligo(dT) Promoter Primer Kit 900375, Waltham, MA) in the first-strand cDNA synthesis reaction. .. Prior to use in a Microarray analysis, the purified mRNA ( ̃1 to 2 μg) can be first reverse transcribed using a T7-Oligo(dT) Promoter Primer (Affymetrix GeneChip T7-Oligo(dT) Promoter Primer Kit 900375, Waltham, MA) in the first-strand cDNA synthesis reaction. .. Next an RNase H-mediated second-strand cDNA synthesis can be performed, the double-stranded cDNA can be purified, and this double-strand cDNA serves as the template in a subsequent in vitro transcription (IVT) reaction (Affymetrix GeneChip IVT kit 900688, Waltham, MA).

    Article Title: Methods, compositions and devices for treating cancer with illudofulvenes
    Article Snippet: Prior to use in a Microarray analysis, the purified mRNA ( ̃1 to 2 μg) can be first reverse transcribed using a T7-Oligo(dT) Promoter Primer (Affymetrix GeneChip T7-Oligo(dT) Promoter Primer Kit 900375, Waltham, Mass.) in the first-strand cDNA synthesis reaction. .. Prior to use in a Microarray analysis, the purified mRNA ( ̃1 to 2 μg) can be first reverse transcribed using a T7-Oligo(dT) Promoter Primer (Affymetrix GeneChip T7-Oligo(dT) Promoter Primer Kit 900375, Waltham, Mass.) in the first-strand cDNA synthesis reaction. .. Next an RNase H-mediated second-strand cDNA synthesis can be performed, the double-stranded cDNA can be purified, and this double-strand cDNA serves as the template in a subsequent in vitro transcription (IVT) reaction (Affymetrix GeneChip IVT kit 900688, Waltham, Mass.).

    Article Title: Inhibitors of Differentiation (ID1, ID2, ID3 and ID4) genes are neuronal targets of MeCP2 that are elevated in Rett syndrome
    Article Snippet: A clean up of RNA was performed using RNeasy Mini kit (QIAGEN) before proceeding to cDNA synthesis. .. 1 μg of RNA under each condition was first reverse transcribed using T7-oligo (dT) promoter primer followed by RNase-H mediated second-stranded cDNA synthesis using One-Cycle cDNA Synthesis kit (Affymetrix). ..

    Labeling:

    Article Title: Translation Elongation Factor eEF1A2 is a Novel Anticancer Target for the Marine Natural Product Plitidepsin
    Article Snippet: Total RNA was obtained from four samples of each HeLa and HeLa-APL-R cells with TRIzol reagent (Life technologies) under the recommendations of the manufacturer. .. Once purified, 15 μg RNA from each sample were reverse transcribed with a T7-Oligo(dT) promoter primer in the first-strand cDNA synthesis reaction, as indicated in the GeneChip One-Cycle Target Labeling Kit protocol (Affymetrix, Santa Clara, CA, USA). ..

    Article Title: Evaluation of in vitro macrophage differentiation during space flight
    Article Snippet: .. Briefly, the two round RNA amplification and labeling procedure was performed using the Affymetrix Small Sample Labeling Protocol vII (2xIVT) as follows: 50 ng of total RNA was primed with T7-oligo(dT) promoter primer (Affymetrix, Santa Clara, CA) and reverse transcribed using SuperScript Reverse Transcriptase vII kit (Invitrogen). .. The first round of RNA amplification was performed on the cDNA using the MEGAScript T7 Invitro-Transciption kit (Ambion).

    Article Title: Precision Profiling and Components of Variability Analysis for Affymetrix Microarray Assays Run in a Clinical Context
    Article Snippet: .. Each total RNA sample was then used for a single cDNA synthesis reaction, using the Affymetrix T7-Oligo (dT) promoter primer, which was subsequently transcribed, labeled, fragmented, and hybridized to one U133A array. ..

    Synthesized:

    Article Title: Expression of Ten-m/Odz3 in the fibrous layer of mandibular condylar cartilage during postnatal growth in mice
    Article Snippet: .. Total RNA was mixed with T7-oligo (dT) promoter primer, reverse-transcribed to cDNA, and synthesized to double-stranded cDNA with a GeneChip® Expression 3′-Amplification Reagents Two-Cycle cDNA Synthesis Kit (Affymetrix, Santa Clara, CA, USA). .. The double-stranded cDNA was purified and served as a template in the subsequent in-vitro transcription using a GeneChip® in-vitro transcription labeling kit (Affymetrix).

    Article Title: Urban Particles Elevated Streptococcus pneumoniae Biofilms, Colonization of the Human Middle Ear Epithelial Cells, Mouse Nasopharynx and Transit to the Middle Ear and Lungs
    Article Snippet: The labeling and hybridization protocols used were standardized by e-biogen (e-biogen Inc., Seoul, South Korea) as per GeneChip expression analysis technical manual (Affymetrix, Inc., USA) . .. Briefly, 300 RNA was converted to cDNA using T7-oligo (dt) promoter primer, and through in vitro transcription the cRNA was synthesized from cDNA using Invitrogen kit (Invitrogen, USA). .. The fragmented cDNA was end-labeled through terminal transfer reaction incorporating a biotinylated dideoxynucleotide and hybridized to the GeneChip Human Gene 2.0 ST array (Affymetrix, Inc., USA) .

    Expressing:

    Article Title: Expression of Ten-m/Odz3 in the fibrous layer of mandibular condylar cartilage during postnatal growth in mice
    Article Snippet: .. Total RNA was mixed with T7-oligo (dT) promoter primer, reverse-transcribed to cDNA, and synthesized to double-stranded cDNA with a GeneChip® Expression 3′-Amplification Reagents Two-Cycle cDNA Synthesis Kit (Affymetrix, Santa Clara, CA, USA). .. The double-stranded cDNA was purified and served as a template in the subsequent in-vitro transcription using a GeneChip® in-vitro transcription labeling kit (Affymetrix).

    RNA Amplification:

    Article Title: Evaluation of in vitro macrophage differentiation during space flight
    Article Snippet: .. Briefly, the two round RNA amplification and labeling procedure was performed using the Affymetrix Small Sample Labeling Protocol vII (2xIVT) as follows: 50 ng of total RNA was primed with T7-oligo(dT) promoter primer (Affymetrix, Santa Clara, CA) and reverse transcribed using SuperScript Reverse Transcriptase vII kit (Invitrogen). .. The first round of RNA amplification was performed on the cDNA using the MEGAScript T7 Invitro-Transciption kit (Ambion).

    Microarray:

    Article Title: Methods, compositions and devices for treating cancer with illudofulvenes
    Article Snippet: Prior to use in a Microarray analysis, the purified mRNA ( ̃1 to 2 μg) can be first reverse transcribed using a T7-Oligo(dT) Promoter Primer (Affymetrix GeneChip T7-Oligo(dT) Promoter Primer Kit 900375, Waltham, MA) in the first-strand cDNA synthesis reaction. .. Prior to use in a Microarray analysis, the purified mRNA ( ̃1 to 2 μg) can be first reverse transcribed using a T7-Oligo(dT) Promoter Primer (Affymetrix GeneChip T7-Oligo(dT) Promoter Primer Kit 900375, Waltham, MA) in the first-strand cDNA synthesis reaction. .. Next an RNase H-mediated second-strand cDNA synthesis can be performed, the double-stranded cDNA can be purified, and this double-strand cDNA serves as the template in a subsequent in vitro transcription (IVT) reaction (Affymetrix GeneChip IVT kit 900688, Waltham, MA).

    Article Title: Methods, compositions and devices for treating cancer with illudofulvenes
    Article Snippet: Prior to use in a Microarray analysis, the purified mRNA ( ̃1 to 2 μg) can be first reverse transcribed using a T7-Oligo(dT) Promoter Primer (Affymetrix GeneChip T7-Oligo(dT) Promoter Primer Kit 900375, Waltham, Mass.) in the first-strand cDNA synthesis reaction. .. Prior to use in a Microarray analysis, the purified mRNA ( ̃1 to 2 μg) can be first reverse transcribed using a T7-Oligo(dT) Promoter Primer (Affymetrix GeneChip T7-Oligo(dT) Promoter Primer Kit 900375, Waltham, Mass.) in the first-strand cDNA synthesis reaction. .. Next an RNase H-mediated second-strand cDNA synthesis can be performed, the double-stranded cDNA can be purified, and this double-strand cDNA serves as the template in a subsequent in vitro transcription (IVT) reaction (Affymetrix GeneChip IVT kit 900688, Waltham, Mass.).

    In Vitro:

    Article Title: Urban Particles Elevated Streptococcus pneumoniae Biofilms, Colonization of the Human Middle Ear Epithelial Cells, Mouse Nasopharynx and Transit to the Middle Ear and Lungs
    Article Snippet: The labeling and hybridization protocols used were standardized by e-biogen (e-biogen Inc., Seoul, South Korea) as per GeneChip expression analysis technical manual (Affymetrix, Inc., USA) . .. Briefly, 300 RNA was converted to cDNA using T7-oligo (dt) promoter primer, and through in vitro transcription the cRNA was synthesized from cDNA using Invitrogen kit (Invitrogen, USA). .. The fragmented cDNA was end-labeled through terminal transfer reaction incorporating a biotinylated dideoxynucleotide and hybridized to the GeneChip Human Gene 2.0 ST array (Affymetrix, Inc., USA) .



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    Comparison of gene expression changes as determined by Affymetrix  GeneChip  analysis and real time RTPCR

    Journal: BMC Immunology

    Article Title: Transcriptional networks in plasmacytoid dendritic cells stimulated with synthetic TLR 7 agonists

    doi: 10.1186/1471-2172-8-26

    Figure Lengend Snippet: Comparison of gene expression changes as determined by Affymetrix GeneChip analysis and real time RTPCR

    Article Snippet: Purified RNA was reverse transcribed using SuperScript Double Stranded cDNA Synthesis kit (Invitrogen Corp, Gaithersburg MA) using a GeneChip ® T7-Oligo(dT) Promoter Primer Kit (Affymetrix Inc. Santa Clara CA) according to manufacturer's recommendations.

    Techniques: Expressing

    Cluster analysis of 680 genes regulated in expression by 3M-852A and 3M-011 . Gene expression analysis was performed at 4 hr post-stimulation of flow-purified pDC with 3M-852A and 3M-011 using the Affymetrix GeneChip U133A as described in Methods. D1 and D2, designate two different donors for pDC preparation in which the pDC purity was > 99%. Global gene expression was determined using the Affymetrix U133A GeneChip. Two-way hierarchical clustering was performed as described in the Methods section, using the Unweighted Pair-Group Method with Arithmetic mean (UPGMA) and the Euclidean similarity measure. The log2 fold change values were used for the analysis. Insert bar chart shows the expression change scale with red, green, and white signifying increased, decreased, and unchanged expression, respectively. Expression changes were evaluated with respect to vehicle stimulated pDC from the same donor. Expression changes for the 680 genes are documented in Additional File .

    Journal: BMC Immunology

    Article Title: Transcriptional networks in plasmacytoid dendritic cells stimulated with synthetic TLR 7 agonists

    doi: 10.1186/1471-2172-8-26

    Figure Lengend Snippet: Cluster analysis of 680 genes regulated in expression by 3M-852A and 3M-011 . Gene expression analysis was performed at 4 hr post-stimulation of flow-purified pDC with 3M-852A and 3M-011 using the Affymetrix GeneChip U133A as described in Methods. D1 and D2, designate two different donors for pDC preparation in which the pDC purity was > 99%. Global gene expression was determined using the Affymetrix U133A GeneChip. Two-way hierarchical clustering was performed as described in the Methods section, using the Unweighted Pair-Group Method with Arithmetic mean (UPGMA) and the Euclidean similarity measure. The log2 fold change values were used for the analysis. Insert bar chart shows the expression change scale with red, green, and white signifying increased, decreased, and unchanged expression, respectively. Expression changes were evaluated with respect to vehicle stimulated pDC from the same donor. Expression changes for the 680 genes are documented in Additional File .

    Article Snippet: Purified RNA was reverse transcribed using SuperScript Double Stranded cDNA Synthesis kit (Invitrogen Corp, Gaithersburg MA) using a GeneChip ® T7-Oligo(dT) Promoter Primer Kit (Affymetrix Inc. Santa Clara CA) according to manufacturer's recommendations.

    Techniques: Expressing, Purification